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ACIDIPHILIUM MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Medium for Acidiphilium sp. DSM 100189
* Modification: Replace glucose with 1.8 g/l D-galactose and add 7.0 g/l FeSO4 x 7 H2O to the autoclaved medium from a 1 M stock solution (pH adjusted to 1.8 using 2 N H2SO4 and sterilized by filtration). Adjust pH of complete medium to 2.5.
Growth conditions: 2-3 days
30 °C
Main sol. 269 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| (NH4)2SO4 | 2.0 | g | 2 | 15.136 | |
| KCl | 0.1 | g | 0.1 | 1.341 | |
| K2HPO4 | 0.5 | g | 0.5 | 2.871 | |
| MgSO4 x 7 H2O | 0.5 | g | 0.5 | 2.029 | |
| Yeast extract | 0.3 | g | 0.3 | - | |
| D-Glucose* | 1.0 | g | 1 | 5.551 | |
| Fe SO4 x 7 H2O* | 7.0 | g | - | - | |
| D-Galactose* | 1.8 | g | - | - | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Autoclave glucose and yeast extract separately as a 10% and 3%, resp., stock solution. Adjust pH of complete medium to 3.0 with 1 N H2SO4. | |||||
Metadata
| Last modified: | 24.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | * 2.5 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Molecular composition
* modified
beta
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| FeSO4 x 7 H2O | 7 | 25.179 |
| (NH4)2SO4 | 2 | 15.136 |
| D-Galactose | 1.8 | 9.991 |
| K2HPO4 | 0.5 | 2.871 |
| MgSO4 x 7 H2O | 0.5 | 2.029 |
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